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. Author manuscript; available in PMC: 2017 Mar 27.
Published in final edited form as: Nano Lett. 2016 Jun 1;16(7):4062ā€“4068. doi: 10.1021/acs.nanolett.6b00650

Figure 1.

Figure 1

Localization of the C and N termini of talin Dimers. (A) A schematic of the GFP-talin-mCherry construct (GTC). GFP is attached to the N-terminal of talin and mCherry to the C-terminal of Talin. The Nā€“C terminal distance (termed stretch) is calculated by averaging the length of two strands of the dimer, that is, (y1 + y2)/2. The distance between the two N-termini is referred to as x. (B) The GTC construct was transfected into talin (āˆ’/āˆ’) cells. Images were processed by the previously described methods for subsequent single molecule identification and tracking. Lower panel represent the illustration of centroid-based localization of a single talin dimer.