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. 2017 Mar 1;23(3):146–155. doi: 10.1089/ten.tec.2016.0492

FIG. 4.

FIG. 4.

Characterization of sprayed platelet fibrin patch on post-MI hearts. (A) Schematic design of sprayed labeled platelet fibrin patch in a mouse model of MI. Animals were euthanized at subsequent time point and hearts were harvested for fluorescent image and sliced for histology; (B) Animals were sacrificed at day 0, day 4, and 7, gross pictures showed a uniform platelet fibrin patch labeled with Texas Red-X epifluorescence ester on the surface of the MI heart at subsequent time points; (C) cryosection of the heart enabled ready identification of the platelet fibrin patch by Texas Red-X epifluorescence at day 0. Scale bar =200 μm; (D) Heart cryosections were stained with 4′, 6-diamidino-2-phenylindole (DAPI) for nuclei, and alpha-sarcomeric actin (alpha-SA) for cardiomyocytes, (arrows) indicating sprayed gel was infiltrating into the myocardium since day 4. Scale bar = 100 μm; (E) To calculate the percentage of degradation over time, the sprayed gel area at days 4 and 7 were measured and then normalized to the day 0 sprayed gel area; (F) Quantitation of cardiomyocytes integrated by the sprayed patch at different time points. MI, myocardial infarction.