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. Author manuscript; available in PMC: 2018 Mar 1.
Published in final edited form as: Neurotoxicol Teratol. 2016 Oct 29;60:40–49. doi: 10.1016/j.ntt.2016.10.012

Figure 4. Phsp-4::gfp expression in L1 larvae with or without concurrent treatment with rapamycin and isoflurane.

Figure 4

A. Phsp-4::gfp expression at t=0 hours (after removal from anesthetic chamber) without isoflurane exposure. B. Phsp-4::gfp expression at t=2 hours (after removal from anesthetic chamber) with isoflurane exposure. C. Phsp-4::gfp expression at t=0 hours (after removal from anesthetic chamber) without isoflurane exposure with rapamycin treatment. D. Phsp-4::gfp expression at t=2 hours (after removal from anesthetic chamber) after 6.5% isoflurane exposure with rapamycin exposure. All plates had 0.2% DMSO on plates to facilitate uptake of rapamycin by the animals. The presence of DMSO mildly increased Phsp-4::gfp expression in the absence of isoflurane. E. Chemotaxis indices (CIs) in adults after exposure to isoflurane as L1 larvae during concurrent treatment with rapamycin. Unexposed animals (dark fill), exposed animals (light fill) and delta values (Δ) for control (left) and rapamycin (right) groups are shown. For all graphs, error bars denote SEM values, N>800 animals for each value. These results show that concurrent treatment with rapamycin completely inhibits AIN in C. elegans.