Figure 5.

A. Protocol timeline used to test for neurotoxicity and preconditioning in C. elegans. First stage larva (L1) are first exposed to isoflurane at their EC₉₅ at age zero hours (arrow) and then again at four to eight hours (blue bar). They are then allowed to grow to adulthood (3 days for wildtype, D3). Chemotaxis is then tested as described in the methods and in Figure 1. B. Chemotaxis Index showing the neurotoxic effect in wildtype (N2) C. elegans following isoflurane exposure (black and gray bars). Preconditioning eliminated the neurotoxic effect caused by isoflurane (cross hatched bar). p value compares the difference between preconditioned animals and non-preconditioned animals exposed to isoflurane (cross hatched and gray bars). Error bars are SEM.