Figure 5. Enforced expression of HCELL on A-hMSCs confers functional E-selectin binding activity equivalent to that of BM-hMSCs.

(A–B): Parallel-plate flow chamber analysis of tethering and rolling interactions of BM-hMSCs (A) and A-hMSCs (B) on E-selectin-expressing endothelial cells. Untreated and FTVII-treated hMSCs were perfused into a parallel-plate flow chamber seeded with TNFα-stimulated HUVEC and their tethering/rolling capacity was then determined at shear stresses of 0.5, 1, 2, 4, 8 and 16 dynes/cm². Both hMSC types exhibited similarly increased tethering/rolling adhesive interactions on HUVECs at shear stress levels of up to 16 dynes/cm² after FTVII treatment. Values are reported as mean ± SEM. Data are representative of three independent experiments. Abbreviations: FTVII, Fucosyltransferase FTVII; HCELL, Hematopoietic Cell E/L-Selectin Ligand; BM-hMSCs, Bone Marrow-derived human Mesenchymal Stem Cells; A-hMSCs, Adipose-derived human Mesenchymal Stem Cells; HUVEC, Human Umbilical Vein Endothelial Cells.