Table 1.
Immunophenotypic profile of surface antigens on hMSCs derived from bone marrow (BM-hMSCs) or adipose tissue (A-hMSCs)
| Surface Markers |
BM-hMSCs | A -h MSCs | ||||
|---|---|---|---|---|---|---|
| % | MFI | % | MFI | p Value % | p Value MFI | |
| CD13 | 92 ± 2 | 19 ± 2 | 99 ± 1 | 25 ± 5 | 0.176 | 0.436 |
| CD29 | 99 ± 1 | 91 ± 11 | 99 ± 1 | 66 ± 17 | 0.830 | 0.108 |
| CD34 | 3 ± 1 | 3 ± 1 | 28 ± 6* | 9 ± 2 | 0.040 | 0.056 |
| CD36 | 10 ± 3 | 2 ± 1 | 69 ± 8* | 19 ± 3* | 0.002 | 0.052 |
| CD44 | 97 ± 2 | 219 ± 44 | 98 ± 1 | 312 ± 78 | 0.840 | 0.314 |
| CD45 | 2 ± 1 | 3 ± 1 | 0.2 ± 0.1 | 4 ± 1 | 0.034 | 0.569 |
| CD49d | 97 ± 3 | 93 ± 2 | 98 ± 1 | 176 ± 20 | 0.727 | 0.055 |
| CD62E | 2 ± 1 | 3 ± 1 | 0.5 ± 0.1 | 3 ± 1 | 0.099 | 0.670 |
| CD73 | 98 ± 3 | 88 ± 22 | 95 ± 3 | 101 ± 15 | 0.752 | 0.727 |
| CD90 | 97 ± 2 | 132 ± 9 | 94 ± 5 | 192 ± 35 | 0.709 | 0.146 |
| CD105 | 97 ± 1 | 64 ± 5 | 97 ± 1 | 77 ± 16 | 0.710 | 0.550 |
| CD106 | 87 ± 2 | 50 ± 3* | 82 ± 1 | 5 ± 1 | 0.136 | 0.001 |
| CD146 | 74 ± 7 | 22 ± 1 | 70 ± 3 | 21 ± 2 | 0.424 | 0.621 |
| CD166 | 98 ± 2 | 94 ± 2 | 97 ± 1 | 79 ± 17 | 0.685 | 0.323 |
| HECA452 | 0.9 ± 0.1 | 3 ± 1 | 2 ± 1 | 3 ± 1 | 0.283 | 0.491 |
Flow cytometry histograms were compared between BM-and A-hMSCs based on their expression of a panel of cell surface markers including those associated with stromal, endothelial, hematopoiteic cells and ligands of E-selectin. The values represent percentage surface positive and MFI (mean ± SEM). Data are representative of experiments performed on BM-and A-hMSC cultures derived from at least twenty individuals.
Statistically significant differences were calculated using paired t-test (*p<0.05).
Abbreviations: BM-hMSCs, Bone Marrow-derived human Mesenchymal Stem Cells; A-hMSCs, Adipose-derived human Mesenchymal Stem Cells; MFI, Mean Fluorescence Intensity.