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. Author manuscript; available in PMC: 2018 Apr 1.
Published in final edited form as: Stem Cells. 2017 Jan 19;35(4):1053–1064. doi: 10.1002/stem.2559

Figure 2. PRL2 deficiency impairs T cell development following hematopoietic stem cell transplantation.

Figure 2

(A) The frequency of donor-derived cells in spleen, thymus, and bone marrow of recipient mice was determined 18 weeks following bone marrow transplantation. Prl2−/− cells show decreased engraftment in thymus compared to Prl2+/+ cells. (B) The frequency of donor-derived cells in the peripheral blood (PB) of recipient mice was determined every 4 weeks following transplant Prl2+/+ or Prl2−/− HSCs into lethally-irradiated recipient mice. CD3+ T cell recovery was significantly lower in recipients repopulated with Prl2−/− HSCs than that of the Prl2+/+ HSCs. (C) Absolute number of donor derived LT-HSCs in 2 femurs (left panel). Absolute number of PB T cells generated from Prl2−/− HSCs was remarkably reduced compared with that of the Prl2+/+ HSCs. (D) Achievement of donor-derived chimerism in each differentiation stage at 16 weeks after transplantation is shown. (E) Representative flow cytometry plot of donor-derived ETPs. Number indicated the percentage of ETPs in total CD45.2 positive cells. (F) The frequency of different T cell population in donor-derived cells is shown (***P<0.001, **P<0.01, n=8).