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. 2017 Mar 3;174(8):700–717. doi: 10.1111/bph.13731

Figure 1.

Figure 1

Effect of IM on the expression of CESs in human hepatoma HepG2 cells and Huh7 cells. (1) The cytotoxicity of IM in HepG2 cells (A) and Huh7 cells (D). Hepatocytes were treated with indicated concentrations of IM for 24 h, and MTT assay was used to measure cell viability. (2) The effect of IM on the CES1 and CES2 mRNA expression in HepG2 cells (B) and Huh7 cells (E). Hepatocytes were treated with indicated concentrations of IM for 24 h. Total RNA was isolated and subjected to qRT‐PCR analysis. The signals from each target were normalized to the signal from GAPDH. (3) The effect of IM on the CES1 and CES2 protein expression in HepG2 cells (C) and Huh7 cells (F). Hepatocytes were exposed to indicated concentrations of IM for 24 h, and the expression of CESs was evaluated by Western blot. Data are expressed as mean ± SEM (n = 5). *P < 0.05, significantly different from control.