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. 2017 Mar 3;174(8):700–717. doi: 10.1111/bph.13731

Figure 7.

Figure 7

The role of PXR in the IM‐induced suppression of CES1 and CES2 expression. (A–D) The effect of PXR overexpression on the suppression of CES1 and CES2 by IM. HepG2 cells were transfected with PXR construct or the corresponding vector (pFlag‐CMV‐2) for 24 h and then treated with or without 2 μM IM for 24 h. Cell lysates were prepared to analyse the expression of CES1, CES2, PXR, CYP3A4 and P‐gp by Western blot respectively. (E–H) The effect of PXR knockdown on the expression of CES1 and CES2, compared with the effect of IM. HepG2 cells were transfected with siRNA‐PXR or the siRNA‐control for 24 h and then treated with or without 2 μM IM for another 24 h. Cell lysates were prepared and subjected to Western blot to determine the expression of CES1, CES2, PXR, CYP3A4 and P‐gp respectively. Data are expressed as mean ± SEM (n = 5). *P < 0.05, significantly different from control group (vector or siRNA‐control transfected and non‐IM pretreated); #P < 0.05, significantly different from vector‐transfected cells.