Figure 4.

(A) Recordings showing the time course of raloxifene‐induced relaxation in endothelium‐denuded aortic rings in control and in the presence of 10 μM actinomycin D (ACT‐D) or 10 μM cycloheximide (CHX). (B) Inhibitory effect of actinomycin D or cycloheximide on raloxifene (1 μM)‐induced aortic relaxation. (C) The maximal relaxant effect of raloxifene in the absence and presence of actinomycin D/cycloheximide. Statistical differences are indicated by * (P < 0.05) between vehicle control and raloxifene group and ^a (P < 0.05) between raloxifene and other treatment groups. Results are mean ± SEM of 6–11 rings from different rats.