bpV(pis) reduces the infarct volume in MCAO rats through PTEN inhibition and ERK1/2 activation. (A) Diagram shows the experimental procedures in MCAO rats. All groups of animals were subjected to two i.c.v. injections. Akt Inhibitor IV (100 μM, 2 μL) or U0126 (500 μM, 2 μL)] was given at 30 min after reperfusion as the first injection, and bpV(pis) (100 μM, 5 μL) was given at 60 min after reperfusion as the second injection.(B) Sample images and the summarized data of TTC staining indicate that bpV(pis) reduces the infarct volume in the rat brain 24 h after ischaemia onset and that pretreatment with Akt Inhibitor IV and/or U0126 block the bpV(pis)‐induced reduction in infarct volume (n = 10 for each group; *P < 0.05, one‐way ANOVA, followed by the Bonferroni post hoc test). I/R, ischaemia–reperfusion.