Table 4.
SSB Linker Length Does Not Affect the Helicase Activity of RecBCD a
| Rate (nM bps s−1)b | Relative ratec | |
|---|---|---|
| wtSSB | 124 ± 1 | 1.00 |
| hisSSB | 114 ± 9 | 0.92 |
| hisSSBΔC8 | 163 ± 10 | 1.31 |
| hisSSB155 | 89 ± 22 | 0.71 |
| hisSSB145 | 101 ± 6 | 0.82 |
| hisSSB133 | 139 ± 5 | 1.12 |
| hisSSB125 | 99 ± 1 | 0.80 |
| gp32 | 148 ± 20 | 1.19 |
a
Assays were carried out as described in Materials and Methods. Three assays were done on the same day.
b
The rate of DNA unwinding is calculated as described.39
c
Rate of DNA unwinding are normalized to the control, wild type SSB.