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. 2017 Mar 1;26(4):834–846. doi: 10.1002/pro.3132

Figure 6.

Figure 6

Shark and Sus GAPs compared with their variants with regards to GTP hydrolysis on Rab7a·GTP and Rab11a·GTP. (A) Protein purification of Shark and Sus GAPs with their variants; the purity is identified by SDS‐PAGE. (B) GAP assay of Shark GAP and its arginine (R437) and glutamine (Q474) variants using Rab7a·GTP as the substrate. (C) Rab11a·GTP as the substrate to determine the GAP activity of Shark GAP and variants. (D) GAP assay of Sus GAP and its arginine (R400) and glutamine (Q437) variants using Rab7a·GTP as the substrate. (E) GAP activity of Sus GAP and variants on Rab11a·GTP.