Figure 3.

The inhibition of mTOR precludes the H. pylori-promoted HIF-1α induction while Akt inhibition is only partially effective. (A,B) AGS cells were pre-treated with Rapamycin (50 nM) for 30 min and then infected with H. pylori 26695 at MOI 100 for 4 h (A) or 8 h (B). The levels of HIF-1α, p-S235/236-S6, S6, and β-actin were analyzed by western blotting. The relative levels of HIF-1α were determined (mean ± SEM; n = 3–4; ^***p < 0.001). (C,D) AGS cells were pre-treated with Akt inhibitor (1 μM) for 30 min and then infected with H. pylori 26695 at MOI 100 for 4 h (C) or 8 h (D). The levels of HIF-1α, p-S473-Akt, total Akt, and β-actin were analyzed by western blotting. The relative levels of HIF-1α were determined (mean ± SEM; n = 3.4, ^*p < 0.05; ^***p < 0.001).