Figure 5.

Effect of HIF-1α silencing on gene induction in response to H. pylori infection. AGS sh-Scr C2 and sh-HIF-1α C7 cells were infected with H. pylori 26695 at MOI 100 for 8 and 24 h. mRNA levels of GLUT-1 (A), LDHA (B), VEGFA (C), and HIF-1α (D) were evaluated by RT-qPCR. The levels of each mRNA were normalized to RNA of the RPS13 housekeeping gene. All data were expressed relative to values obtained for time 0 h of infection in sh-Scr cells (mean ± SEM; n = 3–4, ^*p < 0.05; ^**p < 0.01; ^***p < 0.001).