Fig. 3.

Podometric estimations. Representative glomeruli at an “Immature stage,” mature stage (<38 weeks’ gestation) and mature stage (≥38 weeks’ gestation) are shown by GLEPP1 peroxidase staining (upper panels) and TLE4 red immunofluorescence (lower panels). Podocytes were identified by their having both GLEPP1-positive cytoplasm and TLE4-positive nuclei. Nonspecific TLE4 positive staining was observed in the interstitial compartment in some cases. Podocyte nuclear number, podocyte nuclear mean caliper diameter, GLEPP1-positive area and total glomerular tuft area were measured using software, as outlined in Materials and methods