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. 2017 Mar 28;11:88. doi: 10.3389/fncel.2017.00088

Figure 1.

Figure 1

MiR-384 regulates encephalomyelitis (EAE) pathogenesis. (A) Quantitative RT-PCR analyses of miR-384 expression in peripheral blood (PB) naïve CD4+ T cells sorted from control (ctrl) and EAE mice on day 21 after immunization (n = 3 mice per group). Relative miRNA expression levels were evaluated by the 2−△△ct method and normalized to the expression of U6. The control was set as 1. (B) Mean EAE scores of lentivirus vector (LV)-transfected mice were recorded daily (n = 5 mice per group). (C) Quantitative RT-PCR analyses of miR-384 levels in CD4+ T cells from lymph nodes (LN) and PB of LV-transfected mice 7 days later (n = 3 mice per group). (D) Body weights of LV-transfected mice normalized to the initial body weight of each mouse (n = 5 mice per group). Data are presented as means ± standard deviation (SD). *p < 0.05, **p < 0.01 and ***p < 0.001. Data shown are single representative results from three independent experiments. LV-ctrl, control miR; LV-miR-384, miR-384 sequence; and LV-inhibitor, miR-384-inhibitor.