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. 2017 Mar 28;11:88. doi: 10.3389/fncel.2017.00088

Figure 2.

Figure 2

MiR-384 enhances demyelination in the spinal cord during EAE. EAE mice were sacrificed on day 21 after immunization for the following analyses. Sham controls, mice immunized without myelin oligodendrocyte glycoprotein (MOG) peptide. (A) Representative luxol fast blue (LFB) staining of spinal cords. Boxed areas in left images are enlarged in the images on the right. Scale bars, 50 μm. (B) Quantification of demyelination in (A). (C) Transmission electron micrographs of spinal cords. Arrows show myelin sheath damage. Scale bars, 2 μm. (D) Analysis of g-ratios in (C). (E) Quantitative PCR analyses of Myelin basic protein (MBP) mRNA expression in spinal cords (n = 3 mice per group). Relative expression of mRNA was evaluated by the 2−△△ct method and normalized to the expression of β-actin. The control was set as 1. (F,G) Western blotting analyses of MBP in spinal cords (n = 3 mice per group). (H) MBP immunofluorescence staining of spinal cords. Boxed areas in left images are enlarged in right images. Scale bars, 50 μm. (I) Statistical analyses of mean fluorescence intensity (MFI) of MBP in H (n = 5 per group). Data are presented as means ± SD. *p < 0.05 and **p < 0.01. Data shown are single representative results from three independent experiments.