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. 2017 Mar 28;8:368. doi: 10.3389/fpls.2017.00368

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Copyright © 2017 Miao, Mao, Wang, Liu, Zhang, Li, Chang, Reynolds, Wang and Jing.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Nucleotide polymorphisms and functional marker development of TaSnRK2.3-1A. (A) Four single nucleotide polymorphisms (SNPs) were detected in the non-exon regions of TaSnRK2.3-1A. (B) A dCAPS marker 2.3AM1 was developed based on SNP-1898 (C/T) in the upper segment. Digestion of the amplified 231 bp fragment with HhaI produced fragments of 202 bp/29 bp for accessions with SNP-1898C, yet unacted for accessions with SNP-1898T. Similarly, a CAPS marker 2.3AM2 from SNP-2905 (A/G) was also developed in the lower segment. M, 100 bp DNA Ladder.