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. 2017 Mar 28;8:368. doi: 10.3389/fpls.2017.00368

Table 1.

Primers used for genomic fragment isolation, sequencing and marker development.

Primer set Nucleotide sequence (5′ to 3′) Experimental purpose
GF GTTTGCTCTGAGTTGGTGCTTC
GR CAGTAATAATGTACACAGCGATAG A, B, and D genomic fragment amplification
M13F TGTAAAACGACGGCCAGT
M13R CAGGAAACAGCTATGACC
SeqF1 ACTCGTTAGTCGTTTGATAAGTT
SeqF2 GGGCGATTCAGTGAAGAT
SeqR1 CATGTGACATCTCAGAACCAT Sequencing primers for TaSnRK2.3
AGF TTCACAGTCGGTTTCGTTCG A genome specific target fragment amplification
BGF TAAGTACCGCTTATGACAATCTGTGGTT B genome specific target fragment amplification
AG-HhaI-F TACAACATAGAACTTTAGTAATGGACAGCG
AG-HhaI-R TCACGCCGCAGGACCAA Marker 2.3AM1 developed for SNP-1898 (C/T)
AG-XbaI-F CTTCAAGGAGCCCGAGACG
AG-XbaI-R TTCTTGTTTCAGCAAACCGTACTC Marker 2.3AM2 developed for SNP-2905 (A/G)
BG-N1aIII-F AACTCTGATCTGAACACGAATGCA
BG-N1aIII-R GTGATCCTCTTCAGAGTTTCAGACAC Marker 2.3BM1 development for SNP-2153 (C/T)
BG-AvaII-F AAGGCAGAAAACTTTGAATCATAAC
BG-AvaII-R TCCTCATCGCTCTGCAGCTCGGCCAGGTC Marker 2.3BM2 development for SNP-2638 (C/G)