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Box plots of observed species and phylogenetic diversity for the four primer pairs used in this study, 68f/518r, 341f/785r, 799f/1193r, 967f/1391r. Average number of observed species was based on 97% sequence similarity cut-off value. Averages were calculated based on a rarefied depth of 1,000 sequences per sample, across the replicates for each primer pair. Different letters denote significant differences (Kruskal Wallis, p < 0.05).
