Table 2.

Percentage of sequences with last 3′ base mismatch and PrimerProspector score.

Primer pair	Last base mismatch (%)	Average score ± SE
341f	0.92	0.07 ± 0.0005
518r	0.41	0.08 ± 0.0005
785r	0.79	0.1 ± 0.0006
1193r	0.48	0.21 ± 0.0006
967f	0.34	0.32 ± 0.0007
799f	5.29	0.37 ± 0.001
68f	3.19	0.71 ± 0.002
1391r	4.08	0.87 ± 0.002

The SILVA SSU non-redundant database release 123 was used for the analyses, which contains 597,607 bacterial sequences.