Fig. 8.
Bouton overlap of L6 cells is dependent on soma separation but independent of ∆ori. Relationship between bouton overlap and soma separation for L6 cell pairs. a 1 BOI was higher for closer pairs whereas for larger soma separation (>2 mm), no bouton overlap was observed. The definition of BOI is shown in d (see also “Materials and methods”). a 2 The same data as in a 1, except that pairs with larger than 2 mm separation are excluded. BOI was negatively correlated with soma separation. Gray curve represents fitted exponential (R 2 = 0.29, p < 0.00001, N = 90, decay constant of the fit: 472 µm). b BOI was independent of orientation difference (∆ori) of parent somata (R 2 = 0.002, p = 0.68). c BOI did not differ among iso-, oblique-, and cross-orientation pairs (p = 0.11 for iso vs oblique; p = 0.19 for iso vs cross; p = 0.22 for oblique vs cross). d Calculation of BOI. For a pair of cells, A and B, BOIA→B and BOIB→A were calculated separately, which typically resulted in two different BOI values. In this illustrated case, BOIA→B = 50 % and BOIB→A = 33 %. Each pixel corresponds to 21.28 × 21.28 μm2