FIGURE 1.

Overview of Zm-chlH mutants. (A) Sites of Mu insertions. The nucleotide position of each insertion with respect to the start codon is indicated. The Zm-chlH-3 allele has an 11 bp deletion flanking the insertion. The sequences flanking each insertion are shown below the map, with the 9 bp target site duplications underlined. (B) Yellow phenotype of Zm-chlH mutants. Plants were grown for approximately 8 days in soil. (C) Immunoblot analysis of core subunits of photosynthetic complexes. AtpB is a subunit of the ATP synthase, PsaD is a subunit of PSI, PsbD is a subunit of PSII, and PetD is a subunit of the cytochrome b₆f complex. A single blot was probed sequentially with each antibody. The Ponceau S stained blot below illustrates the abundance of RbcL (the large subunit of Rubisco) and also serves as a loading control.