Figure 3.

TRPC6 deletion protected primary cortical neurons from glutamate- or NMDA-induced neuronal death.(A) Representative images using propidium iodide (PI) staining in WT and TRPC6^−/− primary neurons with glutamate (100 μM) or NMDA (50 μM) pretreatments, respectively. The small, bright condensed nuclei (<2 μm in diameter) (arrow) show the dying neurons. Scale bar, 10 μm. (B,C) Cell death after glutamate and NMDA pretreatment in WT and TRPC6^−/− neurons. ^*P < 0.01 (n = 3 independent experiments/group). In each experiment, three wells and >100 neurons/well were counted.