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. 2017 Mar 28;8:531. doi: 10.3389/fmicb.2017.00531

FIGURE 3.

FIGURE 3

2D-PAGE analysis of the outer membrane and periplasmic proteins of the wild-type (colored orange) and cj0694 and cj1289 mutants (colored blue). Protein samples were prepared as described in Section “Materials and Methods” and separately resolved by 2D-PAGE. Orange spots represent proteins found in the wild-type fractions only, and absent in the mutant fractions. Blue spots represent proteins found in mutant fractions only, and absent in the wild-type fractions. Black spots represent proteins found in both the wild-type and mutant fractions. In (A,C), the circled numbered protein spots in the 2D-gels of the OMs were identified by mass spectrometry analysis. These are: 1, MOMP (PorA); 2, Cj0964 (Mascot score 88); 3, Cj0112 (TolB, Mascot score 2115); 4, Cj1228 (HtrA, Mascot score 2056). In (B,D), the circled numbered protein spots in the periplasms were correlated with those published in our previous study (Hitchcock et al., 2010). These are: 1, TorA; 2, MfrA; 3, Peb1A; 4, Cj0998; 5, Cj0715; 6, Cj1153; 7, Cj1659 (p19 protein).