Figure 6.

Activity of leptospirosis organ extracts on the cleavage of FXa and thrombin-specific substrates. Lungs, kidneys, and livers extracts of Leptospira-infected hamsters at day 8 or non-infected (ni.) animals were mixed with the substrates S-2765 (A) or S-2238 (B) in buffer containing calcium for thrombin and FXa activity, respectively. Controls containing FXa and thrombin were used. After 16 h incubation at 37°C, the cleavage of the chromogenic substrates was measured. The values represent the mean ± standard deviation of triplicates and are representative of two independent experiments.