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. 2017 Mar 28;8:500. doi: 10.3389/fmicb.2017.00500

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Copyright © 2017 Vieira, de Andrade, Morais, Vasconcellos, Dagli and Nascimento.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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L. interrogans cells role as phospholipidic surface for the assembly of the prothrombinase complex. Lung, kidney, and liver extracts of Leptospira-infected hamsters at day 8 or non-infected (ni.) animals were mixed with FXa, FVa, and leptospires as source of phospholipidic surface in buffer containing calcium. A positive control lacking leptospires and containing PSPC was used. After 30 min incubation at 37°C, FII was added and its conversion to thrombin was measured as cleavage of the chromogenic substrate (OD = 405 nm) at different time points (A). In (B), the bars represent the absorbance obtained 30 min after FII addition. The values represent the mean ± standard deviation of triplicates and are representative of two independent experiments.