Table 4.
α-diversity summary and significance of mineralogy and treatment on α-diversity.
| Surfacea | CP-Limited | C-Amended | P-Amended | CP-Amended | ||||
|---|---|---|---|---|---|---|---|---|
| Species richness (S) | Shannon diversity (H') | Species richness (S) | Shannon diversity (H') | Species richness (S) | Shannon diversity (H') | Species richness (S) | Shannon diversity (H') | |
| Calcite | 362 | 6.38 | 195 | 2.41 | 332 | 6.49 | 534 | 6.53 |
| Limestone | 289 | 6.20 | 149 | 3.74 | 326 | 6.57 | 561 | 5.28 |
| Dolostone | 318 | 6.10 | 129 | 3.69 | 337 | 6.69 | 435 | 4.14 |
| Basalt | 71 | 3.04 | 115 | 5.21 | 332 | 6.55 | 323 | 4.27 |
| Quartz | 55 | 3.67 | 54 | 3.80 | 332 | 6.68 | 548 | 5.23 |
| Albite | 57 | 4.96 | 210 | 2.57 | 329 | 6.65 | 547 | 5.78 |
| Microcline | 116 | 6.33 | 190 | 2.27 | 301 | 6.43 | 381 | 5.00 |
| Chert | 0 | 0 | 143 | 2.93 | 300 | 6.18 | 542 | 5.55 |
| Planktonic | 133 | 5.79 | 120 | 1.90 | 63 | 2.24 | 424 | 5.65 |
| Whole Reactor | 349 | 6.63 | 386 | 3.56 | 459 | 6.54 | 757 | 5.63 |
| Mean ± s.e. | 175 ± 127 | 5.31 ± 1.30 | 145 ± 49 | 3.17 ± 1.04 | 295 ± 88 | 6.05 ± 1.44 | 477 ± 88 | 5.27 ± 0.74 |
| Surface correlation factorsb | F (P) | F (P) | F (P) | F (P) | F (P) | F (P) | F (P) | F (P) |
| Buffering capacity | 7.6 (0.024) | 3.2 (0.036) | 1.1 (0.328) | 0.8 (0.461) | 0.9 (0.461) | 0.7 (0.566) | 0.8 (0.399) | 0.1 (0.892) |
| Mineral type | 5.6 (0.556) | 1.1 (0.624) | 3.9 (0.606) | 2.2 (0.420) | 0.4 (0.996) | 0.3 (0.991) | 0.6 (0.996) | 0.2 (0.884) |
| Mineral phosphate | 0.7 (0.461) | 0.18 (0.834) | 0.2 (0.863) | 4.3 (0.007) | 0.9 (0.602) | 1.0 (0.428) | 1.3 (0.201) | 1.7 (0.061) |
| Treatment correlation factorsb | Species richness F (P) | Shannon diversity F (P) | ||||||
| Carbon Amendment | 1.1 (0.278) | 3.1 (0.006) | ||||||
| Phosphate Amendment | 6.9 (0.0001) | 3.2 (0.004) | ||||||
| Media pHin | 2.8 (0.011) | 3.2 (0.003) | ||||||
| Media pHout | 1.1 (0.301) | 0.3 (0.758) | ||||||
| Treatmentsc | Species richness F (P) | Shannon diversity F (P) | ||||||
| CP-Limited vs. C-Amended | 0.4 (0.892) | 3.2 (0.048) | ||||||
| P-Amended vs. CP-Amended | 0.1 (0.887) | 1.7 (0.656) | ||||||
| P-Amended vs. C-Amended | 5.1 (0.002) | 4.2 (0.008) | ||||||
| P-Amended vs. CP-Limited | 3.6 (0.042) | 1.8 (0.709) | ||||||
| CP-Limited vs. CP-Amended | 4.5 (0.005) | 0.7 (0.997) | ||||||
| C-Amended vs. CP-Amended | 7.2 (0.001) | 3.7 (0.006) | ||||||
Measures of species richness (S) and Shannon Diversity (H') for each surface and each treatment. Values are based on rarefied data sets for comparison across treatments.
Impact of surface factors and treatments assessed by PERMANOVA. Surface factors are buffering capacity (high vs. low based on surface as a carbonate or non-carbonate), mineral type (carbonate, silicate, aluminosilicate, planktonic), and mineral phosphate (high vs. low). Treatment factors are carbon amendment (yes vs. no), phosphate amendment (yes vs. no), media pHin (high vs. low), and media pHout (high vs. low). Values are pseudo-F ratio (F) and the level of significance (P). P < 0.05 indicate a significant difference in a factor (shown in bold).
Pairwise comparisons by treatment use PERMANOVA.