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. 2017 Mar 28;8:169. doi: 10.3389/fphys.2017.00169

Figure 2.

Figure 2

CA assays on purified bovine CA II. (A) Time courses of pH from 0 to 200 s, for concentrations of bCAII ranging from 0 to 4 μg/ml in the reaction cell. We added bCAII to solution A (Table 1) and derived pH-values from the fluorescence of pyranine, added to solution B. (B) Time courses from 0 to 5 s, representing the same records as in (A). (C) Dependence of the rate constant of the pH change on [bCAII]RxCell. We obtained kΔpH-values from non-linear least-squares curve fits of data like those in (A,B). (D) Effect of acetazolamide on time courses of pH from 0 to 200 s for [bCAII]RxCell ranging from 0 to 4 μg/ml. These experiments are like those in (A), except that we added 20 μM ACZ to solution B (Table 1). (E) Time courses from 0 to 5 s, representing the same records as in (D). (F) Dependence of kΔpH on [bCAII]RxCell in the presence of ACZ. Each data point represents mean ± SD (error bar not shown if smaller than the symbol). N represents the number of independent experiments, each covering six concentrations (0–4 μg/ml).