Figure 4.

Role of Microbiota of P2rx7^−/− Mice in Altering Glucose Metabolism

(A–E) Body (A) and WAT (B) weight variation, blood glucose (C) and insulin levels (D), and GTT (E) in WT and P2rx7^−/− mice after 14 days of VAM (n = 5).

(F–I) Weight gain (F), body and WAT weights (G), blood glucose and insulin (H), and GTT (I) in WT mice transplanted with WT or P2rx7^−/− microbiota (n = 5).

(J) Concentrations of ATP in ilea from GF mice either non-colonized (GF) or colonized with pBAD28 or pHND10 bearing E. coli.

(K) Quantification of Tfh and GC B cells in non-colonized or monocolonized animals as indicated.

(L) Intestinal anti-E. coli IgA quantification at fluorescence-activated cell sorting (FACS) (see Experimental Procedures) in GF mice or mice monocolonized with the indicated E. coli transformants (n = 5).

Means ± SEM are shown, and Mann-Whitney test (A–D, G, H, and J–L) and two-way ANOVA (E, F, and I) were used. ^∗p < 0.05, ^∗∗p < 0.01, ^∗∗∗p < 0.001.