Table 2.
Protein | D (m ± SE) μm2/s | Temperature (°C) | Method | Rh–effnm | Reference |
---|---|---|---|---|---|
DDR | 115 ± 11a | 25 | PIPE | 2.4 ± 0.2 | this work |
EGFP | 95 | 22.5 | sFCS | 2.42 | Petrášek and Schwille (2008) |
EGFP | 94b | 22 | FCS | 2.42 | Schenk et al. (2004) |
GFP | 87 | 25 | FCS | 2.82 | Terry et al. (1995) |
GFP | 130 | 20 | FRAP | 1.66c | Busch et al. (2000) |
EGFP | 87 | RT | FRAP | Swaminathan et al. (1997) |
sFCS, scanning fluorescence correlation spectroscopy.
This value was extrapolated from the Stokes-Einstein relation measured using glycerol titration. The error reflects the range of parameter values of the linear regression used to fit the Stokes-Einstein relation, within 2σ confidence intervals.
The original value of 63 μm2/s was corrected after publication.
The authors verified the Stokes radius using dynamic light scattering (DLS).