Figure 1. Effect of primer concentration on the discrimination of Salmonella typhimurium and Vibrio parahaemolyticus target amplification by different T_m values generated by melting curve analysis of the multiplex LAMP assay.

(A) Equal concentrations of Salmonella and V. parahaemolyticus primers. (B) Halved concentrations of Salmonella primers. The melting curve shows temperature on the X-axis and fluorescence on the Y-axis. Well 1 (purple), S. typhimurium; well 2 (red), V. parahaemolyticus; well 3 (yellow), S. typhimurium and V. parahaemolyticus; well 4 (green), negative control.