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. 2017 Mar 28;7:45601. doi: 10.1038/srep45601

Figure 2. Sensitivity of the multiplex LAMP compared with multiplex PCR for the simultaneous detection of Salmonella typhimurium and Vibrio parahaemolyticus.

Figure 2

10-fold serial dilutions of S. typhimurium (CICC 21483) and V. parahaemolyticus (ATCC 17802) DNA templates from 100 ng/μL to 0.1 pg/μL were tested. (A) The amplification curve generated from 100 ng/μL to 10 pg/μL shows time on the X-axis and fluorescence on the Y-axis. (B) Two melting peaks (84.62 ± 0.18 °C for S. typhimurium and 81.90 ± 0.23 °C for V. parahaemolyticus) distinguishing S. typhimurium and V. parahaemolyticus amplification products were generated by melting curve analysis. (C) Sensitivity of the multiplex PCR for simultaneous detection of S. typhimurium and V. Parahaemolyticus. Lanes 1–7: S. typhimurium and V. parahaemolyticus DNA concentrations of 100 ng/μL, 10 ng/μL, 1 ng/μL, 100 pg/μL, 10 pg/μL, 1 pg/μL and negative control.