Figure 6. NavBeta1 effect on hKv1.3-del ionic currents.

(A) Representative ionic currents from hKv1.3-del alone (black, n = 5), co-injected with rNavBeta1 (red, n = 4) or co-injected with hNavBeta1 (blue, n = 4). Pulse protocol is shown at the top of the panel. (B) Normalized conductance as a function of voltage (G-V) for hKv1.3-del alone (black), co-injected with rNavBeta1 (red) or with hNavBeta1 (blue). (C) Time constants of activation as a function of voltage for hKv1.3-del alone (black), co-injected with rNavBeta1 (red) or co-injected with hNavBeta1 (blue). Time constants were obtained as weighted average values from the fit of the rising face of the currents (highlighted in yellow in A) to two exponential functions. Blue star (*) indicates statistical significance (p-value < 0.05) of the difference between hKv1.3-del alone (black) and with hNavBeta1 (blue) from +10 mV to +80 mV except at +20 mV. (D) Deactivation time constants from the tail currents at –80 mV (highlighted in yellow at the end of the pulse in A) for hKv1.3-del alone (black), co-injected with rNavBeta1 (red) or co-injected with hNavBeta1 (blue). Time constants were obtained as weighted average values from the fit to two exponentials. Stars (*) indicate statistical significance (p-value < 0.05) of the difference between hKv1.3-del alone (black) and with rNavBeta1 (red) in red, and with hNavBeta1 (blue) in blue, from −20 mV to +80 mV. (E) Comparison between G-V and Q-V between hKv1.3 alone and when co-injected with rNavBeta1. To reach 50% of the maximum conductance (G_max), hKv1.3 with rNavBeta1 requires less charge movement than hKv1.3 alone, indicating rNavBeta1 may strengthen the coupling between VSD movement and pore opening.