FIGURE 3.
Characterization of the influence of various thiols on the interaction of hnRNP-E1 with the 25-nucleotide hnRNP-E1 mRNA cis element and the accumulation of various thiol amino acids in placental 1584-LF compared with 1584-HF cells. Gel-shift assays on the interaction of 0.5 μg dialyzed purified recombinant GST–hnRNP-E1 with 1 × 105 counts/min of [35S]25-nucleotide hnRNP-E1 mRNA cis element in the presence of various indicated thiols (A). The result is representative of 2 studies that gave comparable results. Comparison of the percentage increase in various thiols in placental 1584-LF over 1584-HF cells (B); the data were taken from Table 5 to highlight the percentage increase in homocysteine over other thiols in folate-depleted cells. *Different from control, P < 0.05. GST, glutathione S-transferase; HF, high folate; hnRNP-E1, wild-type heterogeneous nuclear ribonucleoprotein E1; LF, low folate; nt, nucleotide; β-Me, β-mercaptoethanol.