. 2017 Mar 1;147(4):482–498. doi: 10.3945/jn.116.241364

TABLE 2.

Dose-dependent influence of iron(II) on the K_D involving the l-homocysteine–triggered RNA-protein interaction between the [³⁵S]25-nucleotide hnRNP-E1 mRNA cis element and purified recombinant GST–hnRNP-E1 protein¹

Protein	Iron(II), μM	l-homocysteine, μM	K_D, nM
GST–hnRNP-E1	0	25	2.74 ± 0.52^a
GST–hnRNP-E1	5	25	3.72 ± 0.34^b
GST–hnRNP-E1	25	25	6.50 ± 0.83^c

Values are means ± SDs, n = 3 (means of triplicates). Labeled means without a common superscript letter differ, P < 0.05. GST, glutathione S-transferase; hnRNP-E1, wild-type heterogeneous nuclear ribonucleoprotein E1; iron(II), ferrous sulfate heptahydrate; K_D, dissociation constant.