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. 2017 Mar 1;147(4):482–498. doi: 10.3945/jn.116.241364

TABLE 8.

Evidence of the specificity of RNAi against hnRNP-E1 mRNA in perturbing the biosynthetic rate of newly synthesized hnRNP-E1 and folate receptor proteins in placental 1584-HF cells1

RNAi transfected in placental 1584-HF cells [35S]cysteine–hnRNP-E1, fmol · mg protein−1 · h−1 [35S]cysteine–folate receptor, fmol · mg protein−1 · h−1
Wild-type 25-nucleotide hnRNP-E1 RNA 1.02 ± 0.07a 1.58 ± 0.02a
Scrambled 25-nucleotide RNA 0.95 ± 0.04a 1.57 ± 0.05a
RNAi against 25-nucleotide hnRNP-E1 mRNA 0.32 ± 0.02b 0.35 ± 0.02b
1

Values are means ± SDs, n = 3 (means of triplicates). Means in a column without a common superscript letter differ, P < 0.05. HF, high folate; hnRNP-E1, heterogeneous nuclear ribonucleoprotein E1; RNAi, RNA interference.