FIGURE 2.

Purine biosynthesis in WT and ADH5 KO HepG2 cells. (A) Silencing of ADH5 was confirmed by immunoblotting. (B) The ratio of [¹⁴C]-formate to [³H]-hypoxanthine indicates the incorporation of formate into DNA via the de novo purine synthesis pathway relative to the incorporation of hypoxanthine into DNA via the purine salvage pathway. Labeled means without a common letter differ, P < 0.05. (C) Total cellular folate concentrations. *Different from folate-sufficient cells, P < 0.05. Data were analyzed by using 2-factor ANOVA with Bonferroni corrections. Values are means ± SDs of 3 biological replicates/condition. ADH5, alcohol dehydrogenase 5; DPM, decays per minute; KO, knockout; WT, wild-type.