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. 2016 Dec 31;25(2):392–400. doi: 10.1016/j.ymthe.2016.11.017

Figure 2.

Figure 2

CHIP Overexpression Diminishes Neuronal Degeneration in the CA1 Region after Global Brain Ischemia, Followed by 7 Days of Reperfusion in the Hippocampus

(A–D) Representative photomicrographs of FluoroJade C-positive cells in the CA1 region of sham-operated rats (A), rats submitted to global brain ischemia followed by 7 days of reperfusion (GBI) (B), rAAV8-733-GFP-infected rats submitted to GBI (C), or rAAV8-733-CHIP-infected rats submitted to GBI (D). (E) Quantification of FluoroJade C-positive cells in the CA1 region per millimeter in different experimental conditions. Tukey’s post-test was performed for this analysis. 95% confidence interval (CI); **p < 0.001; NS, non-significant (p > 0.5). Error bars indicate ± SEM. (F–H) Representative photomicrographs of immunofluorescence for cleaved caspase-3-positive cells in the CA1 region of sham-operated rats (F), rAAV8-733-GFP-infected rats submitted to GBI (G), and rAAV8-733-CHIP rats submitted to GBI (H). n = 3–5 animals per group; 40× magnification. Scale bar indicates 50 μm.