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. 2016 Dec 31;25(2):392–400. doi: 10.1016/j.ymthe.2016.11.017

Figure 3.

Figure 3

CHIP Overexpression Prevents eIF2α Phosphorylation Induced by Oxygen and Glucose Deprivation after 24 hr of Reperfusion In Vitro

(A) Western blots for p-eIF2α (Ser51) and total eIF2α from homogenates of organotypic hippocampal slices under different experimental conditions. (B) Quantification of normalized optical density for p-eIF2α and total eIF2α bands. α-tubulin was used as loading control. n = 3 independent experiments from a pool of hippocampal slices taken from nine rats. 95% confidence interval (CI); *p < 0.05; **p < 0.01; NS, non-significant. Error bars indicate ± SEM. N, normoxia; OGD, oxygen and glucose deprivation for 90 min followed by 24 hr of reperfusion; GFP+OGD, rAAV8-733-GFP-infected slices submitted to OGD; CHIP+OGD, rAAV8-733-CHIP-infected slices submitted to OGD.