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. 2017 Mar 28;9(9):477–486. doi: 10.4254/wjh.v9.i9.477

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©The Author(s) 2017. Published by Baishideng Publishing Group Inc. All rights reserved.

This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial.

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Western blot analysis of hepatitis B surface antigen (A, C), hepatitis B virus core (B), core related proteins (D) expressed by hepatitis B virus genotype D clones (WT) and hepatitis B surface antigen mutant clones (120T and 143L) in the supernatant of Huh 7 cells. The results for the wild type clones in the three independent transfection experiments were similar and their mean value is set at 1.0. The hepatitis B surface antigen and hepatitis B virus core proteins levels are expressed relative to this value in (C) and (D). HBV: Hepatitis B virus.