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. 2017 Mar 28;7:45159. doi: 10.1038/srep45159

Figure 8. Both HPV16 L1 and HPV16 L2 interact with VPS4WT-GFP and VPS4EQ-GFP during virus infection.

Figure 8

The 293-VPS4WT-GFP and 293-VPS4EQ-GFP cells were either untreated (−) or induced (+) with 1 μM ponA and after 24 h were infected with HPV-16 PsVs. At 6 h and 9 h post infection the cells were harvested and cell extracts incubated with rabbit anti-GFP antibody overnight at 4 °C. The next day immunoprecipitates were collected on protein A conjugated sepharose beads for 1 h at room temperature. The co-immunoprecipitating L1 and L2 proteins were then detected by western blotting using mouse anti-L1 and mouse anti-L2 antibodies (top panel). The lower panel shows the inputs for HPV16 L1, HPV16 L2 and VPS4 (GFP).