Table 1. IC50 values (μM) relative to the specified POPC and DOTAP ruthenium-containing liposomes, and to cisplatin (cDDP), used as cytotoxic reference drug, and to the naked AziRu complex in the listed breast cancer cell lines following 48 h of incubation.
| Cell lines | IC50 (μM ± SEM) | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| ToThyRu/POPC | DoHuRu/POPC | ToThyRu/DOTAP | DoHuRu/DOTAP | AziRu | cDDP | |||||
| Total liposome | Ru | Total liposome | Ru | Total liposome | Ru | Total liposome | Ru | |||
| MCF-7 | 185 ± 0.1 | 27.8 ± 0.1 | 126 ± 0.1 | 18.9 ± 0.1 | 74.6 ± 0.1 | 10.1 ± 0.1 | 67.3 ± 0.2 | 10.3 ± 0.2 | >250 | 17 ± 1 |
| MDA-MB-231 | 239 ± 3 | 35.8 ± 3 | 98 ± 1 | 14.7 ± 1 | 79.5 ± 0.2 | 10.8 ± 0.2 | 81 ± 0.3 | 12.1 ± 0.3 | >250 | 19 ± 1.5 |
| MDA-MB-436 | >250 | >37.5 | 245 ± 1 | 36.7 ± 1 | 110 ± 0.2 | 15.0 ± 0.2 | 130.9 ± 0.2 | 20.0 ± 0.2 | >250 | NA |
| MDA-MB-468 | 111.1 ± 0.1 | 15.7 ± 0.1 | 136 ± 0.8 | 20.4 ± 0.8 | 107.8 ± 0.1 | 14.7 ± 0.1 | 95 ± 0.1 | 14.2 ± 0.1 | >250 | 24 ± 1 |
| CG-5 | 138 ± 0.2 | 19.4 ± 0.2 | 204 ± 0.8 | 30.6 ± 0.8 | 30.1 ± 0.2 | 4.1 ± 0.2 | 21.5 ± 0.2 | 3.3 ± 0.2 | >250 | NA |
The ruthenium IC50 values corresponding to the effective metal concentration (15% mol/mol) carried by each nanoaggregate are reported as mean values ± SEM (n = 30). (NA = not assessed).