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. 2017 Mar 28;7:45408. doi: 10.1038/srep45408

Figure 5. IOX2-mediated inhibition of PHD2 promotes differentiation of articular chondrocytes.

Figure 5

Primary articular chondrocytes were cultured with 20 μM PHD2 inhibitor IOX2 or vehicle (DMSO) for 72 hours and expression of markers were measured by Real time RT-PCR. (A) mRNA expression of cell proliferation marker p57Kip2 and cyclin D1. (B) mRNA expression of SZ progenitor marker tenascin C and clusterin. (C) mRNA expression of chondrocyte marker Col2 and chondrocyte hypertrophy marker Col10. (D) mRNA expression of HIF-1α targets, vascularization marker Vegf, Epo, and glycolysis marker Glut1 and Pgk1. *P < 0.05, **P < 0.01, n = 4/group. Data were normalized to controls and presented as the mean ± SEM.