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. 2017 Mar 28;7:45287. doi: 10.1038/srep45287

Figure 3. S009-131 induced DNA damage response through activation of PIKKs.

Figure 3

(A) HCT116 cells were treated with 7.5 μM of S009-131 for indicated time points. Whole cell lysates were then collected and analysed by immunoblotting using antibodies specific to ATM, ATR and DNA-PK and their active phosphorylated form. (B–D) Cells were incubated with S009-131 with or without pre-treatment with specific PIKK inhibitors (KU5993, 10 μM; NU7441, 10 μM and VE821, 5 μM) and analysed for γ-H2AX expression by immunoblotting.