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Stacked qHNMR spectra (DMSO-d6; 600 MHz) of the red clover extract and the isoflavones were divided into four key regions representing different spin systems present in the isoflavones. 6, prunetin; 5, calycosin; 4, daidzein; 3, genistein; 2, formononetin; 1, biochanin A; RCE, red clover extract. The NMR spin parameters for PS, PB, and IR were obtained from MetIDB. Depending on the type of the spin system, the A-ring protons fall into either region 2 (AAXX′) or 3 (AMX).
