Figure 6.

Evaluation of DUX4 mRNA expression in mouse tibialis anterior muscles co-injected with rAAV-D4Z4/pLAM virus and either pLAM3A or control vivo-PMO. The TAs of the mice were injected with 1E11 DRP of D4Z4/pLAM AAV virus and 10 µg of vivo-PMO per leg. The mice were sacrificed 10 days post-injection, and total RNAs were extracted with Trizol. Reverse transcription was performed on 800 ng of DNase-treated total RNA with the 3’ adaptor of the RLM-RACE kit (Ambion) and 2 μL of the resulting cDNA were amplified by nested PCR for 50 cycles in total. The RT-PCR products were analysed by electrophoresis on a 1.5% agarose gel and stained with ethidium bromide.