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. 2017 Jan 9;25(2):379–391. doi: 10.1016/j.ymthe.2016.12.010

Figure 5.

Figure 5

RWM Injection of Exo-AAV1-HA-Lhfpl5 Induces LHFPL5 Bundle Expression in Hair Cells and Rescues FM1-43 Loading

(A) HA-LHFPL5 detected with immunolabeling for the HA tag. Cochleas from Lhfpl5−/−mice (C57BL/6 background) were injected through the round window at P1 with exo-AAV1-CBA-HA-Lhfpl5. Hair bundle actin was stained with phalloidin (red). HA staining is apparent in the IHC and OHC bundles as well as in some supporting cells. P4+2 days in culture. Scale bar, 20 μm. (B) High magnification images show anti-HA staining in the bundles of an inner hair cell and an outer hair cell. Anti-HA staining is detectable at the tips of all rows of stereocilia. Scale bar, 2 μm. (C) RWM injection of exo-AAV1-CBA-HA-Lhfpl5 through the round window at P1 restores FM1-43 loading in IHCs and OHCs (7 days after injection; P6+2). Scale bar, 20 μm. (D) Regional transduction efficiency based on HA staining in bundles of the apical, middle, and basal regions of the cochlea (P4+2) (n = 4). No difference was apparent between different regions.