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. 2017 Mar 28;11:83. doi: 10.3389/fncel.2017.00083

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Copyright © 2017 Huang, Xia, Liu, Cao, Huang and Luo.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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SC-astrocyte confrontation assay. (A) A schematic drawing showing that magnetized SCs and astrocytes were seeded in parallel strips and were allowed to migrate and intermingle for 1 week under an applied MF. (B–F) Representative images of the SC-astrocyte confrontation assay. (G) Quantification of the confrontation assay. SCs were immunolabeled for P75 (green) while astrocytes were immunolabeled for GFAP (red). (H) Quantification of mean area of astrocytes in contact with SCs. Arrows point in the direction of the MF. Scale bar = 100 μm. Data are expressed as means ± SD; ^**p < 0.01,^*p < 0.05.